tpi [2017-06-06 08:49:23]

triose phosphate isomerase, glycolytic/ gluconeogenic enzyme

Locus: BSU33920

Isoelectric point: 4.79

Molecular weight: 26.88 kDa

Protein length: 253 aa

Gene length: 759 bp

Function: enzyme in glycolysis/ gluconeogenesis

Product: triosephosphate isomerase

Essential: no

E.C.: 5.3.1.1

Synonyms: tpiA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Carbon core metabolism → Glycolysis
• Metabolism → Carbon metabolism → Carbon core metabolism → Gluconeogenesis
• Information processing → Genetics → Newly identified competence genes
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Gene

Coordinates: 3,479,405 → 3,480,166

Phenotypes of a mutant

• essential PubMed, non-essential according to PubMed

The protein

Catalyzed reaction/ biological activity

• D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate (according to Swiss-Prot)

Protein family

• triosephosphate isomerase family (according to Swiss-Prot)

Modification

• phosphorylation on Ser-213 PubMed

Effectors of protein activity

• inhibited by 2-phosphoglycolate (in B. stearothermophilus) PubMed

Structure

• 1BTM (complex with 2-phosphoglycolic acid, Geobacillus stearothermophilus), complex with 2-phosphpoglycolic acid, Geobacillus stearothermophilus NCBI

Localization

• cytoplasm (homogeneously distributed throughout the cell) PubMed

Additional information

• extensive information on the structure and enzymatic properties of Tpi can be found at Proteopedia
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: cggR-gapA-pgk-tpi-pgm-eno
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CggR: repression, PubMed, in CggR regulon

  Regulation

  • expression induced by glycolytic intermediates (CggR) CggR PubMed
  • the mRNA is processed between cggR and gapA by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed

• Genes: pgk-tpi-pgm-eno
  Description: PubMed

Biological materials

Mutant

• GP700 (cat), available in Jörg Stülke's lab, PubMed

Expression vector

• pGP394 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
• pGP89 (N-terminal Strep-tag, for SPINE, expression in B. subtilis), available in Jörg Stülke's lab
• pGP1511 (expression in B. subtilis, in pBQ200), available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

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